Course: FEBS practical course Functional imaging of cellular signals, June 2017

June 11-16, 2017

The ability of cells to perceive and correctly respond to their environment is the basis of development, tissue repair, and immunity as well as normal tissue homeostasis. Errors in cell signaling are responsible for many diseases such as cancer, autoimmunity, and diabetes. By understanding cell signaling, diseases may be treated effectively and, theoretically, artificial tissues may be created. Microscopy techniques can be used to elucidate signal complex composition, organization and dynamics, as this reflects the functions of cells and their organelles.

This course is organized by the Leeuwenhoek Centre for Advanced Microscopy (LCAM) and provides an in-depth view of specific advanced imaging techniques used at imaging centers of AMC, NKI and FNWI. The course focuses fully on intensive hands-on practical sessions and interactive discussions with experts in the various microscope techniques.

This practical advanced course aims to provide 20 young scientists in this field with theoretical background and to give hands-on experience of state-of-the-art microscopy techniques.


  • Confocal microscopy
  • Spinning disc and TIRF microscopy
  • Förster resonance energy transfer (FRET) of biosensors
  • Fluorescence lifetime imaging (FLIM)
  • Fluorescence recovery after Photobleaching (FRAP)
  • Fluorescence Correlation Spectroscopy (FCS)


Target audience: PhD students, postdocs and other interested scientists. Experience with microscopy is a prerequisite for this Advanced Microscopy Course.

Course organizer: Mark Hink


More information and application go to the FEBS course website (click here).