Zooming in on plasmamembrane dynamics 2012

Title: LCAM-ESF course“Zooming in on plasmamembrane dynamics with Advanced Light Microscopy”logo-ESF
Date: 11-15 June 2012
Location: LCAM-FNWI, -NKI & -AMC
Contact: Dorus Gadella & Mark Hink
Previous editions: (In the Footsteps of van Leeuwenhoek)
Sponsors: European Science Foundation (Euromembrane)


Introduction:
This is a special edition of the annual “In the footsteps of van Leeuwenhoek course”
Recent technical developments have sparked a new wave of interest in the field of membrane research. Microscopic techniques can be used to elucidate  membrane composition, organization and dynamics, as this reflects the  functions of cells and their organelles, transport of membranes, transport  across membranes and signaling.  Because  of the recent technological revolution in advanced light microscopy  (super-resolution imaging, functional imaging of molecules) it is now  possible to directly monitor the dynamics of (single) molecules in  biomembranes in living cells. This practical advanced course will provide  students  the  theoretical background and give hands-on experience of state-of-the-art  (membrane) microscopy techniques. 

The program includes:
– Lectures
– Student presentations
– Hands-on practical sessions
– Networking and social programesfcourse12

Program:
The course will have a daily structure consisting of two to three lectures, discussing advanced microscopy techniques and their applications in membrane research. During the practical sessions the students will get hands-on experience, working at the microscopes, being instructed by experts in the field. Because of the restricted capacity at the microscopes this course will allow for 4 groups of 4 students at the practical sessions.

Sunday      June 10th 2012: 
Arrival
Monday      June 11th 2012:

9.00                         Welcoming address (Dorus Gadella)
9.15-10.00                 Lecture confocal microscopy (Erik Manders)
10.00-11.00               Lecture TIRF microscopy (Ulrike Engel)
11.00-11.30               Coffee break
11.30-12.30               Lecture Image correlation spectroscopy (Paul Wiseman)
12.30-13.30               Lunch
13.30-17.00               Practicals confocal/TIRF of 4 groups of 4 students
17.00-19.00               Dinner
19.00-20.30               Flash-presentations from participants

Tuesday June      12th 2012:
9.00-12.30                Practicals confocal/TIRF of 4 groups of 4 students
12.30-13.30               Lunch
13.30-14.30              Lecture fluorescent dyes  and sensors (Carsten Schultz)
14.30-15.30               Lecture fluorescent proteins (Vladislav Verkhusha)
15.30-16.00               Coffee break
16.00-17.00               Lecture fluorescent membrane sensors (Tamas Balla)
18.00-20.00               Dinner

Wednesday      June 13th 2012:
9.00-10.00                 Lecture Fluorescence Lifetime Imaging Microscopy(Dorus Gadella)
10.00-11.00               Lecture FRET in membrane systems (Kees Jalink)
11.00-11.30               Coffee break
11.30-12.30               Lecture Fluorescence Recovery After Photobleaching (Adriaan Houtsmuller)
12.30-13.30               Lunch
13.30-17.00               Practicals N&B/ FLIM/FRET/FRAP of 4 groups of 4 students
18.00-20.00               Dinner

Thursday      June 14th 2012:
9.00-10.00                Lecture FFS techniques for membrane studies (Mark Hink)
10.00-11.00               Lecture FRAP in membrane systems (Eric Reits)
11.00-11.30               Coffee break
11.30-12.30               Lecture Super-resolution microscopy (Thomas Schmidt)
12.30-13.30               Lunch
13.30-17.00               Practicals N&B/ FLIM/FRET/FRAP of 4 groups of 4 students
18.00-20.00               Dinner

Friday June 15th 2012:

9.00-12.30                 Practicals N&B/ FLIM/FRET/FRAP of 4 groups of 4 students
12.30-13.30               Lunch
13.30-16.30               Practicals N&B/ FLIM/FRET/FRAP of 4 groups of 4 students
17.00-17.30               Closing remarks (Dorus Gadella)
18.00-20.00               Dinner

Saturday June      16th 2012:
Departure

 

Top