In the footsteps of van Leeuwenhoek

Title: In the footsteps of van Leeuwenhoek 2016logo_LCAM
Date: June 2016, details later
Location: LCAM-AMC, -NKI and -FNWI
Contact: Eric Reits (
Previous editions: 2009, 2010, 2011 & 2014



This course is organized by the Leeuwenhoek Centre for Advanced Microscopy (LCAM) and provides an in-depth view of specific advanced imaging techniques used at imaging centers of AMC, NKI and  FNWI.  The course focuses fully on intensive hands-on practical sessions and interactive discussions with experts in the various microscope techniques. On the final day participants present their own research topic and discuss which techniques should be implemented in their project.

During the course participants will visit the imaging centres of three institutes in Amsterdam, the NKI (Dutch Cancer Institute), the FNWI (UvA Science Park) and the AMC. In each institute the part will focus in depth on specific advanced imaging techniques.


– Measuring intra- and inter-molecular protein interactions using FRET, FLIM & FCS
– Confocal microscopy methods including spinning disk (CLSM)
– Superresolution microscopy
– Confocal quality control
– Correlative microscopy (bridging fluorescence microscopy and electron microscopy)
– Usage of fluorophores such as FlAsH/ReAsH 

Target audience:

PhD students and postdocs. An introduction course like the Basic Microscopy course is a prerequisite for the Advanced Microscopy Course. For less experienced users a two days startup course will be given the week in advance.

Course organizers:

AMC Ron Hoebe, Eric Reits, Jan Stap, Henk van Veen
FNWI Dorus Gadella, Joachim Goedhart, Mark Hink
NKI Lenny Brocks, Kees Jalink


More information and application:

From Eric Reits / / tel. +31 (0)20 566 6259