{"id":652,"date":"2017-06-02T11:27:26","date_gmt":"2017-06-02T09:27:26","guid":{"rendered":"http:\/\/www.microscopycourse.nl\/?page_id=652"},"modified":"2026-07-15T15:40:06","modified_gmt":"2026-07-15T13:40:06","slug":"lcam-functional-imaging-annual","status":"publish","type":"page","link":"http:\/\/www.microscopycourse.nl\/?page_id=652","title":{"rendered":"LCAM Functional Imaging (annual)"},"content":{"rendered":"
\n

Title: Functional Imaging\"logo_LCAM\"<\/strong>
\n Date: 5-14 October 2026 (annual)<\/strong>
\n Location: FNWI, UMCA-AMC & NKI<\/strong>
\n Contact: LCAM-FNWI<\/strong>
\n Previous editions: 2018-2019 & 2021-2025<\/strong><\/p>\n

Introduction<\/h3>\n

The general aim of this course is to get hands-on experience and theoretical background in performing state-of-the-art advanced microscopy applied to cell biology. Due to the revolution in genetic encoded labeling (GFPs, switchable probes, FlAsH and AGT-tagging, etc) and the revolution in advanced (light) microscopy (confocal, super-resolution, FRET, dynamics etc) it is now not only possible to image the location of fluorescently labeled molecules but with the functional imaging techniques one can quantify the dynamics of molecular processes inside cells with unprecedented resolution, speed and accuracy. Because the application of advanced microscopy is essential for modern research in cell biology, this course provides training in the diverse modes of advanced microscopy. The microscope setups on which the training is provided are part of the van Leeuwenhoek Centre for Advanced Microscopy (LCAM) in Amsterdam, in which the research groups of Molecular Cytology (Swammerdam Institute for Life Sciences, UvA), of Cell Biology and Histology of the University Medical Center Amsterdam (UMCA) and the Cellular Biophysics Group, Dept of Cell Biology Netherlands Cancer Institute (NKI) .<\/p>\n

\"micstudents\"<\/p>\n

Course content:<\/h2>\n

The van Leeuwenhoek Centre for Advanced Microscopy (LCAM) organises the next edition of the “Functional Imaging” course at the University of Amsterdam (UvA), the Netherlands at Oct 5th-16th 2026.<\/p>\n

This 8 day course is an intermediate level course for PhD students, postdocs, labtechnicians & microscopy facility staff in biology and biomedical science who already have experience with widefield and\/or confocal fluorescence microscopy but want to extend their knowledge with the functional imaging techniques. After this course, participants will understand the principles of these methods and their limitations. In this course we will focus on functional imaging technologies that can be used in biology to measure concentrations, mobility, interactions and biosensors such as FRET, FLIM, FRAP, FCS & super-resolution microscopy (STED). Furthermore, participants will be introduced to the latest developments in fluorescent probes and biosensors, sample preparation, data-analysis & data statistics.<\/p>\n

The course consists of theoretical lectures & tutorials (~50%) and hands-on experiments (~50%) at the microscope, including basic image analysis of these data sets. This course runs in parallel with the 4 weeks Advanced Microscopy course for UvA master students. The teaching staff consists of experts in the field from the van Leeuwenhoek Centre of Advanced Microscopy. Two special sessions will be dedicated to discuss each participant\u2019s specific microscopy project & issues and data-analysis questions.<\/p>\n

Participants:<\/b> Min. 4 and Max. 8 PhD students, postdocs and other scientists
\nLevel: <\/b>Experienced microscopist
\nCourse fee:<\/b> 475 \u20ac
\nInfo:<\/b> http:\/\/www.microscopycourse.nl\/?page_id=652<\/a>
\nApplication deadline:<\/b> September 14th 2026
\nSelection criteria:<\/b> Microscopy experience, research project and motivation, to be included in the application form.
\nLocation: <\/b>LCAM microscopy labs in Amsterdam, the Netherlands
\nOrganisers:<\/b> LCAM-FNWI, contact: Mark Hink (m.a.hink@uva.nl<\/a>)<\/p>\n<\/div>\n

Program:<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Mon<\/td>\nFluorescence microscopy & probes (summary)<\/td>\n<\/tr>\n
<\/td>\nFluorescence Resonance Energy Transfer (FRET) microscopy<\/td>\n<\/tr>\n
Tue<\/td>\nSuper-resolution microscopy (practical focused at STED)<\/td>\n<\/tr>\n
Wed<\/td>\nFluorescence Lifetime Imaging Microscopy (FLIM)<\/td>\n<\/tr>\n
Thu<\/td>\nFluorescence Recovery After Photobleaching (FRAP), fluorescence loss in photobleaching (FLIP) & photoactivation microscopy<\/td>\n<\/tr>\n
Fri<\/td>\nProject discussion session with each participant<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/tr>\n
Weekend<\/td>\nNo course elements<\/td>\n<\/tr>\n
Mon<\/td>\nFluorescence Correlation Spectroscopy (FCS)<\/td>\n<\/tr>\n
Tue<\/td>\nFluorescence Recovery After Photobleaching (FRAP), fluorescence loss in photobleaching (FLIP) & photoactivation microscopy<\/td>\n<\/tr>\n
Wed<\/td>\nImage processing and statistics for microscopy data<\/td>\n<\/tr>\n
<\/td>\nCustomised data analysis session<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Requirements:<\/strong> Since this is not a basic but intermediate level course the participants should have some experience with widefield and\/or confocal microscopy and be familiar with the following theory and terms: <\/span>
\n<\/span><\/p>\n\n\n\n\n\n\n
– microscopy:<\/td>\nimage formation by a lens\/ magnification \/ Numerical Aperture \/ phase contrast\/ fluorescence \/ filter cube
\n\/ pinhole \/ scanning vs camera imaging \/ confocal vs widefield microscopy<\/td>\n<\/tr>\n
– detectors:<\/td>\nPMT \/ CCD camera<\/td>\n<\/tr>\n
– images & data:<\/td>\npixels \/ 8-bit, 12-bit\/RGB \/ LUT \/ brightness \/ contrast<\/td>\n<\/tr>\n
– image analysis:<\/td>\nbasic experience with image analysis using Image J, Imaris or similar.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

Date: <\/strong>Total 8 working days Mon 5 – Wed 14 October 2026 (excluding the weekend)
\n<\/strong>Participants: <\/strong>Minimum 4, Maximum 8 PhD students, technicians, postdocs & microscope facility staff.
\nLevel:<\/strong> Experienced microscopist
\nCosts:<\/strong> 475 Euro. Besides full course attendance, the fee includes course materials and use of the microscopes. Attendees will be responsible for their own travel, lodging and meals.
\nApplication:<\/strong> One can apply for the course here<\/a>. The deadline for applications is Sept 14th 2026. At Sep 15th everyone will be notified if the course will take place (enough qualified applicants) and if so who has been selected to participate.
\nSelection criteria:<\/strong> Microscopy experience, research project and motivation, to be included in the application form.
\nOrganiser & info:<\/strong> LCAM-FNWI<\/p>\n

\n


\n<\/strong>
\n<\/strong><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Title: Functional Imaging Date: 5-14 October 2026 (annual) Location: FNWI, UMCA-AMC & NKI Contact: LCAM-FNWI Previous editions: 2018-2019 & 2021-2025 Introduction The general aim of this course is to get hands-on experience and theoretical background in performing state-of-the-art advanced microscopy …<\/p>\n

LCAM Functional Imaging (annual)<\/span> Read More »<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"class_list":["post-652","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/www.microscopycourse.nl\/index.php?rest_route=\/wp\/v2\/pages\/652","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.microscopycourse.nl\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/www.microscopycourse.nl\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/www.microscopycourse.nl\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.microscopycourse.nl\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=652"}],"version-history":[{"count":73,"href":"http:\/\/www.microscopycourse.nl\/index.php?rest_route=\/wp\/v2\/pages\/652\/revisions"}],"predecessor-version":[{"id":1226,"href":"http:\/\/www.microscopycourse.nl\/index.php?rest_route=\/wp\/v2\/pages\/652\/revisions\/1226"}],"wp:attachment":[{"href":"http:\/\/www.microscopycourse.nl\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=652"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}